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Larisa Haupt

Queensland University of Technology

Associate Professor Larisa Haupt is a Principal Research Fellow and the Neurogenesis and Stem Cell Group Leader and Laboratory Manager within the Genomics Research Centre, and Program Leader in Diagnostics and Functional Genomics within the QUT Tier 1 Centre for Genomics and Personalised Health at IHBI. A/Prof Haupt has extensive research expertise in the extracellular matrix, stem cells, cell and molecular biology and human molecular genetics. Her research team has a particular interest in the role of the extracellular matrix, with a focus on the proteoglycans, in the regulation and dysregulation of cell behaviour including lineage specification, neurodegeneration and cancer. A/Prof Haupt and her team utilise molecular and cell biological two- and three-dimensional human stem cell culture models in conjunction with next generation sequencing platforms to unravel these complex mechanisms in humans. In the last 10 years, A/Prof Haupt has published 50 manuscripts (43 in the last 5 years; 13 to date in 2020), with a current Google Scholar H index of 27 and an i10-index of 62. 


In vitro models of human neurogenesis

Larisa M Haupt, Rachel K. Okolicsanyi, Chieh Yu, Lotta E. Oikari, Ian W Peall, Lyn R. Griffiths

Abstract

Assessing the functionality of neuronal cell types is critical to their efficacy in future applications. Understanding how these processes are regulated will help to further unravel the structural complexity of the human brain, and the role of associated biological and other factors in neurogenesis. This information will also have important ramifications for the development of 2D and 3D models to translate cells toward their successful integration of newly formed neurons into existing/remaining neural circuits. The complexity of the neural niche and the ubiquitous presence of the protetoglycan proteins in the neural microenvironment suggest that this will be achievable not by one assay but will more likely be achieved through a combination of data and approaches to identify specific markers and regulatory pathways. Our approach uses human stem cell models and cell lines including human mesenchymal stem cells (hMSC), embryonic derived human neural stem cells (hNSC H9) and ENStem-A neural progenitor cells, as well as the immortalised human neural progenitors (ReNcell CX cell line), in 2D and developing 3D cultures to examine the role of proteoglycans and their use as markers of lineage potential and specification.