• 5 Days of Stem Cells - a virtual event
A Virtual Event
5 Days of Stem Cells
Connect. Discover. Advance.
Join us for the world’s leading virtual stem cell event.
Photo

Jean Lu

Genomics Research Center, Academia Sinica

Research Interests

We use high throughput screening to target chemicals/cytokines/shRNAs those can efficiently promote cell renewal/differentiation/transdifferentiation

Educational Background

2000 Ph.D. Institute of Microbiology, National Taiwan University

1994 M. S. Institute of Molecular Medicine, National Taiwan University

1992 B.S. Department of Medical Technology, National Taiwan University

Professional Experiences

2018-present Adjunct Associate Professor, Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.

2016-present Adjunct Associate Professor, Department of Life Science, Tzu Chi University, Taiwan

2015-present Associate Research Fellow, Stem Cell Program, Genomics Research Center, Academia Sinica, Taiwan

2015-present Adjunct Associate Professor, Genomics and System Biology Program, College of Life Science, National Taiwan University, Taiwan

2003-2007 Postdoctoral Fellow/Associate, Molecular, Department of Cellular, and Developmental Biology, Yale University, USA


A high-throughput functional screen reveals human embryonic stem cell self-renewal signals

Abstract

Only very few studies focus on the cytokines secreted by hESCs. We screened and investigated a chemokine (C-X-C motif) ligand 14 (CXCL14), is a downstream effector of ATF1 is critical for ESC renewal. Disruption of CXCL14 expression downregulate the expression of Oct4/Sox2/Nanog, arrest the cell cycle at G0/G1 stage, and further increased the expression levels of differentiated markers. Furthermore, by co-immunoprecipitation, ELISA, and duo-link assay, we demonstrated that CXCL14 is the ligand for the insulin-like growth factor 1 receptor (IGF-1R). CXCL14 can stimulate IGF-1R signal cascade to maintain hESC self-renewal. For now, the literature indicates that all receptors in the CXCL family belong to G protein-coupled receptors (GPCRs). This study is the first to identify that a CXCL chemokine can bind to and trigger a receptor tyrosine kinase (RTK), IGF-1R. In addition to IGF-1 and insulin, this is also the third ligand of IGF1-R. These findings increase our understanding of signal transduction and stem cell biology.